Abstract :
Loop-Mediated Isothermal Amplification (LAMP) is an upcoming molecular method for TB diagnosis. It uses six primers to amplify DNA isothermally and results can be observed visually. The present study is focused on the comparison of performance of LAMP and PCR targetting IS6110 with conventional methods- smear microcopy and culture. Sputum samples were collected from 230 TB suspects which were analysed by all the above methods. LAMP detected the maximum number of TB cases (111/119) as compared to PCR (97/119), smear (98/119) or culture (105/119) Overall the sensitivity and specificity of LAMP was 93.2% (95% CI: 87.197.0%) and 97.1% (95% CI: 91.8-99.4%) respectively.These results are very encouraging and suggest that LAMP has the potential to serve as efficient method of diagnosis for TB.
Keyword :
PCR, LAMP, IS6110, Mycobacterium tuberculosis