Abstract :
This work deals with the study of anti leishmanial activities of
plant abstracts that have been evaluated for possible anti
leishmanial activities using MTT Assay. In vitro inhibition was
observed with chloroform growth and a similar inhibition was
obtained with aqueous extract of s.nigrum and all other
extracts exhibiting significant toxicity. Promastigotes of L.
donovani strain AG83 were routinely cultured at 24OC in M199
medium supplemented with 10% fetal calf serum, 100 U
penicillin/ml and 100 µg streptomycin/ml. The IC50 of
Pentamidine was found to be 38g/ml while that for
w.somnifera and s.nigrum found to be 50g/ml and 58g/ml
respectively. This paper records one of the effective measures
of cure of the deadly disease by medicinal plants solanum
nigrum and withania somnifera against the recent drug
pentamidine .
Keyword :
L. donovani, anti- leishmaniasis, pentamidine, s. nigrum, w. somnifera, medicinal plants, in-vitro/in-vivo, mitochondrial dehydrogenase, formazan,