Volume :
1
Issue :
2
Abstract :
An efficient and accurate reverse phase – high performance liquid chromatographic method was developed and validated for the separation and determination of synthetic food colorant Tartrazine E 102. This method was successfully applied for the estimation of tartrazine dye in certain food stuff (cream biscuits, Tang, gems, and candies) and in drugs (Aceclofenac and Metoprolol tablets - in which tartrazine (E102) was present as a coating agent). Interaction study was carried out to find the effect of tartrazine on the protein binding of Aceclofenac and metoprolol in Bovine serum albumin. A Phenomenex C 18 Gemini column (150×4.6 mm), 5 µ particle size was used as stationary phase. Mobile phase contained a mixture of 10mM ammonium acetate buffer: Acetonitrile : Methanol in the ratio of (50:25:25v/v/v) at PH 8. The E102 dye was successfully separated out at retention time of 1.299 min, by using isocratic elution technique at room temperature. Photo diode array detector monitored the wavelength of tartrazine dye as 426nm. The flow rate was selected was 1ml/min. The method was thoroughly validated. Limit of detection etection and limit of quantization for tartrazine were found to be 0.1ng and 1 ng ml respectively. The intra-day precision and inter-day precision were determined as 0.72 % RSD and 0.78 % RSD respectively. The objective of this research was to estimate the tartrazine dye in various food stuff and formulation, and to determine the effect of dye on drug. The dye E102 extracted and quantified in various food stuff such as (Tang - 255µg/ml, Cream biscuits - 633µg/ml, Gems – 147µg/ml, Candies - 67µg/ml) and found that tartrazine content was more in cream biscuits compared to other food stuff. The amount of E102present in Aceclofenac tablet was found to be 5.5 mg/ tablet and that of metoprolol tablet was found to be 5.14mg/ tablet. A study on protein binding of aceclofenac and metoprolol by UV spectroscopy and reverse phase high performance liquid chromatography was conducted. The effect of tartrazine on protein binding of aceclofenac in BSA and metoprolol in BSA and were carried out and determined that Protein binding of the drugs was altered by the effect of dye.