Abstract :
Aim: The aim of the present is to develop simple, accurate and precise RP-HPLC method for the quantification of Eugenol content in various tulsi based ayurvedic formulations.
Materials and Methods: HPLC was carried out by reverse –phase technique on a C18 column with a mobile phase composed of Acetonitrile: Methanol in the proportion of 50:50 v/v, at a flow rate of 1.0 mL /min. The detection was carried at 281 nm by UV- Visible detector. The linearity was established in the concentration range of 10-50 mcg/mL with an R value of >0.999. Method and system precision was established with an RSD of < 2>
Results: The method was found to be specific, precise, accurate and robust. The proposed method can be used for the reliable quantification of Eugenol.
Keyword :
Eugenol, Tulsi, RPHPLC, Accuracy, Precision, Ayurvedic formulations