Abstract :
Objective and Design: Beta Cross-linked C-terminal telopeptide of type I collagen (fragment β- CTX) is newer addition to the list of bone resorption markers in serum. The purpose of our study was to estimate and compare the levels β-CTX in gingival crevicular fluid (GCF) and serum of periodontally healthy individuals and chronic periodontitis (CP) patients before and after nonsurgical periodontal therapy (NSPT).
Materials and Methods: Twenty Five subjects with equal distribution of males and females were divided into two groups i.e Group I: 10 Periodontally healthy individuals, Group II: 15 CP patients aged between 30-50 years. GCF and serum samples were collected at baseline in Group I whereas in Group II GCF and serum samples were collected at baseline and 2 months after NSPT. The levels of GCF and serum β-CTX were quantified using enzyme-linked immunosorbent assay (ELISA). The clinical parameters evaluated were plaque index (PI), gingival index (GI), probing pocket depth (PPD) and clinical attachment level (CAL) and the correlations of β-CTX in GCF and serum with clinical parameters were evaluated.
Results: The mean concentration of β-CTX increased from Group I to Group II. The mean concentration of β-CTX in GCF and serum was reduced after 2 months of NSPT. Furthermore, reduction in β-CTX levels was positively correlated to gain in CAL in GCF.
Conclusion: Periodontal treatment resulted in the reduction of β-CTX levels suggesting that β- CTX can be considered as a potential marker of bone resorption in CP.
Keyword :
Collagen, Gingival Crevicular Fluid, C-terminal telopeptide, Chronic Periodontitis, Bone Resorption, Bone turnover Marker.