Abstract :
Context: Aqueous extract of cassava leaves (Manihot esculenta Crantz) has a high rutin content. Rutin has
benefits as an antioxidant, anti-inflammatory, analgesic, and antidiabetic. The plant extract has low bioavailability
and has a large molecular weight. Therefore, a carrier system like niosomes is required to allow aqueous leaves
extract of cassava through to absorb in the body. Aims: The purpose of this study was to determine the effect
of variation concentration of Span 40 that can trap aqueous extract of cassava leaves in niosomes optimally.
Materials and Methods: Niosomes were done by thin-layer hydration method with concentration of Span 40
which was varied into three formulas: Formula A (100 ?mol), Formula B (150 ?mol), and Formula C (200 ?mol).
Statistical Analysis Used: The data analysis using SPSS analyzed by one-way ANOVA. Results: The rutin
contained in cassava leaves of this research is 42.52%. Entrapment efficiency test used dialysis membrane method
and the result showed that the increased concentration of Span 40 is used; the entrapment efficiency is also
increasing. The result of SPSS test using one-way ANOVA of three formulas did not show significant difference
(P > 0.05). Conclusions: Optimum concentration of Span 40 in trapping aqueous extract of cassava leaves is
Formula A (100 ?mol) which was 95.9%.
Keyword :
Cassava leaves, entrapment efficiency, niosomes, rutin, Span 40