Abstract :
The aim of this research is to study the anti-inflammatory activity of ethanolic extract of the leaves of Acacia
eherbengiana (Hayne), using carrageenan induced paw edema model in rats, albumin denaturation and heat induced
hemolysis.
Methods: a total of twelve animals of both sexes weighing (60 - 130 g) were randomly divided into three groups
consisting of 4 animals each. Group I served as control and receive distilled water, while group II and III received the
ethanolic extract of Acacia eherbengiana (300 mgkg body weight) and diclofenac sodium (20mgkg) respectively.
Inflammation was induced by injecting carrageenan (1% wv) in the sub-plantar tissues of the rat hind paw.
In vitro anti-inflammatory activity of the ethanolic extract was evaluated by inhibition of protein denaturation and
human RBCs membrane stabilization methods at 250, 500, 750 and 1000 µg/ml.
Results: the ethanolic extract of Acacia eherbengiana (300 mgkg body weight) produced 40% inhibition of paw
edema volume while diclofenac sodium produced 40.6% inhibition. The percent of membrane stabilization of Acacia
eherbengiana was found to be 57.9% while for diclofenac sodium was 55.8% and that of protein denaturation was
found to be 84.6%, 42.8% for Acacia eherbengiana and diclofenac sodium respectively
Conclusion:
The ethanolic extract of the Acacia Eherbengiana exhibited significant anti-inflammatory activity which was found to
be time dependent
Keyword :
Acacia eherbengiana , anti-inflammatory, carrageenan, albumin denaturation, heat induced hemolysis