Abstract :
Methicillin-resistant coagulase-negative staphylococci (MR-CoNS) associated infection is a growing concern in healthcare settings now a day. MR-CoNS are the main infectious agents of the hospital acquired infection. Clinical staffs to patients transmission of resistant strains have caused a rapid increase in the prevalence of antimicrobial resistance in recent years. Growing rate of antimicrobial resistant against available antibiotics of MR-CoNS is a developing problem in low income or lower middle income counties.
Aim: This study was conducted to determine the occurrence MR-CoNS isolated from different clinical staffs of tertiary care hospital.
Materials and Methods: This prospective study conducted in clinical staffs, nasal swab were collected from all the participants. Screening of CoNS were done on the basis of cultural, morphological and biochemical tests, identification and AST analysis done by VITEK-2 automated system. Methicillin resistance pattern was checked by VITEK-2 and Kirby-Bauer disc diffusion method according to CLSI guideline.
Results: A total of 129 nasal swab samples were collected from clinical staffs, of which n=81 isolates (85.6%) were CoNS. Among n=81 CoNS, (48.12%) S. epidermidis, (41.97%) S. haemolyticus, (7.4%) S. lugdunensis and S. saprophyticus (2.4%) were reported. Out of n=81 CoNS isolates, n=26 were conformed as MR-CoNS. Maximum methicillin resistance were reported in S. epidermidis 53.48% (14/26), S. haemolyticus 42.30% (11/26), S. lugdunensis 3.84% (1/26) and S. saprophyticus 0% (0/26).
Conclusion: The occurrence rate of MR-CoNS are higher (20.6%) in the healthcare workers and most of the methicillin resistant-CoNS isolates shows high level of resistance against widely used antibiotics but all the isolates susceptible against vancomycin.
Keyword :
MR-CoNS, CoNS, Hospital Associated Infection, Clinical staffs, Oxacillin-Cefoxitin screening.