Abstract :
A new stability indicating liquid chromatographic method has been developed for the determination of
Eprosartan and Hydrochlorothiazide in pharmaceutical formulations (gradient mode; UV detection at 235 nm).
Linearity was observed over the concentration range 1-300 μg/ml for Hydrochlorothiazide 19.2-750.3 μg/ml for
Eprosartan respectively with regression equations y = 5980.5x+535.64 (R2 = 0.9999) and y = 8199.2x + 565.86 (R2
= 0.9998) respectively. The combined tablet formulation of Eprosartan and Hydrochlorothiazide was subjected to
acidic, alkaline, oxidation, thermal, photolytic and humidity degradations and the method was validated as per ICH
guidelines. The method was reported to be simple, specific, precise, accurate, robust and useful for the routine
analysis of Eprosartan and Hydrochlorothiazide in pharmaceutical dosage forms.
Keyword :
Eprosartan, Hydrochlorothiazide, RP-HPLC, stability-indicating and validation