Volume :

2

Issue :

3

Abstract :

A simple, precise and accurate spectrophotometric method has been developed for the determination of Abacavir Sulfate nanoparticles. Standard Stock solution was prepared in pH 7.4 phosphate buffer and further dilution were carried out with the same. The λmax of Abacavir Sulfate nanoparticles was found to be 285 nm. The A (1%, 1cm) Value of Abacavir Sulfate nanoparticles was found to be 434. The correlation co efficient (r) was found to be 0.9999. The limit of detection (LOD) and limit of Quantification (LOQ) was found to be 0.1074 μg/ml and 0.3257 μg/ml respectively for Abacavir Sulfate nanoparticles. The result of estimation in Abacavir sulfate nanoparticles was found to be 99.62 ± 0.25%. The method was then validated statistically as per KH guidelines, which yielded good results concerning range, linearity, precision, accuracy specifically, Robustness and Ruggedness.