Abstract :
The mrigal carp Cirrhinus mrigala is an economically important food fish and also an important aquaculture freshwater species. DNA content of fish tissues are of considerable interest for their specificity in relation to food values of fish and evaluating their physiological requirements at different periods of life. Fish exhibit large variations in their biochemical contents from species to species. The present study was conducted on DNA extraction and determination of DNA quantity of both male and female of wild and cultured Cirrhinus mrigala using Nanophotometer. DNA extraction was done and extracted DNA was analyzed using nanophotometer to determine the concentration of DNA. For the present study both male and female of wild Cirrhinus mrigala (from middle Ganga Region) and cultured Cirrhinus mrigala (from culture ponds and/ hatchery) were taken from different sites of Western Uttar Pradesh. The value of DNA concentration in female of wild Cirrhinus mrigala was between 62 - 66 ng/μl and of male was between 64- 78 ng/μl. The value of DNA concentration in female of cultured Cirrhinus mrigala was between 60 - 88 ng/μl and of male was between 70- 78 ng/μl.
Keyword :
DNA extraction, DNA quantification, nanophotometer, Cirrhinus mrigala