Abstract :
To understand the function of Sly-miR393 in tomato, the precursor sequences and potential target genes of
Sly-miR393 were identificated from tomato genome database by computational homology search method. The SlymiR393 gene was amplified from the genomic DNA by PCR and cloned into plant expression vector pLP35s-100. SlymiR393 guided-cleavage to putative target transcripts was validated u sing 5RACE RT-PCR. In this study, our results indicated that the precursor sequence of Sly-miR393 contains the complete hairpin structure. TIR1/AFB auxin receptor genes contain recognition sites with high complementarities to Sly-miR393 sequence. In tomato, Sly-miR393 directs the cleavage of SlTIR1.SlTIR1-like1 and SlAFB mRNA, then auxin receptor homologous was validated to be as target of Sly-miR393. The pLP35s-pre-SlymiR393 vector containing Sly-miR393 gene was successfully constructed, which would provide significant evidence for further study of Sly-miR393 function in auxin signaling pathway in tomato.
Keyword :
Sly-miR393; Target Genes; Auxin Receptor Gene; Construction