Abstract :
Background: Diabetes mellitus is a metabolic disorder which impedes the normal steps of the process of
wound healing. It has higher risks of foot infection and postoperative wound infection that is polymicrobial
with increased antibiotic resistance.
Objectives: A comparative study of bacteriological profile from the wound samples of diabetic and nondiabetic
patients and to understand the relationship between bacterial load and high glycemic index among
diabetic patients.
Materials and Methods: A cross sectional study involving 50 diabetic and 50 non- diabetic patients
wound swab or pus samples collected under aseptic precautions. Results: Polymicrobial infection is
mostly observed. Among the isolates, gram negative bacilli were about 55% and gram positive cocci
were about 45%. The most common organism isolated was Staphylococcus aureus in diabetic and nondiabetic
wound. In diabetic wound the predominant organism isolated was Staphylococcus aureus and
Proteus mirabilis followed by Pseudomonas species. In non- diabetic wound, the predominant organism
isolated was Staphylococcus aureus followed by Pseudomonas species. The Total Extended spectrum beta
lactamase producer was about 76% whereas total Methicillin Resistant Staphylococcus aureus was 23%. In
diabetic wound, glycemic index was poorly controlled in which fasting blood glucose level was > 150mg
and post prandial blood glucose level was > 210mg among majority of cases.
Conclusion: Antibiotic susceptibility test screening showed gram positive cocci isolates to more sensitive
towards Erythromycin, Vancomycin, Amikacin and gram negative bacilli isolates to be more sensitive for
Amikacin, Gentamycin and Imipenem. Thus, early diagnosis of diabetic wound infection is required for
the antimicrobial therapy.
Keyword :
Bacteriological profile, Diabetic wound, Glycemic index, Non diabetic wound.