Abstract :
We aimed to scrutinize the effect of EA@CS-NP on DMBA induced hamster buccal pouch tumorigenesis.
To determine the inflammatory proteins, cell cycle regulatory proteins, angiogenic proteins and apoptotic proteins
(intrinsic and extrinsic) by investigating some important regulators involved in the above mentioned signalling
pathways. The histological microphotographs of DMBA induced hamster buccal pouch (HBP) showed squamous
cell carcinoma with the keratin pearl formation with an increased Immunohistochemical expression of epidermal
growth factor receptor (EGFR) and proliferative protein (Ki-67). Supplementation of EA@CS-NP to tumor bearing
animals disclosed the dysplastic lesions with a decreased staining pattern of EGFR and Ki-67 expression. The protein
appearance pattern such as, fas, fasL, caspase 3, caspase 8, caspase 9, PARP, Bax was found to be down regulated,
perhaps the expression level of p53, Bcl2, cox-2, VEGF & cyclin D1 were markedly unregulated in DMBA induced
positive control group. However, supplementation of EA@CS-NP altered the above mentioned protein expressions
to near normal. Taken together, our results suggest that supplementation of EA@CS-NP inhibited the progression of
HBP tumorigenesis by triggering both intrinsic and extrinsic apoptotic pathways.
Keyword :
Ellagic acid, Chemotherapy, Oral Cancer, Apoptosis, DMBA.