Abstract :
Abstract Aims and Objectives: Bacterial metabolites have shown promise in thetreatment of a wide range of disorders, and they play an essential role in maintaininghuman health. The purpose of this research was to find out how effective Bacillussubtilis bioactive chemical compounds are against bacteria and other microbes.Methods: The bioactive chemical components, also called secondary metabolites,were analysed using gas chromatography-mass spectrometry (GC-MS) methods.Then, in vitro evaluation of the antibacterial activity of the Bacillus subtilismethanolic extract was carried out.Results: The GC-MS analysis of Bacillus subtilis detected the presence of thefollowing: The compounds listed include tert-Butyl 12-aminododecanoate, 1,12-Diaminododecane, Ethylidenehydroxylamine, 5,6-Diamino-2,3-dicyanopyrazine, 1-methylpiperidine-3-carboxylic Acid, 3,3-Dimethyl-2-acetyloxirane, 8-Hexadecanol,4-methyl-hexadecane, 5,7-Octadecadiynoic acid, 4,6-dimethylpyridin-2-amine. Thecurrent study examined the bioactivity of the ethanolic extract of Bacillus subtilisand the standard antibiotics AP-Ampicillin and KN-Kanamycin against five testedpathogens: Staphylococcus saprophyticus (11.67±0.06, 21.07±0.09, and 12.31±0.06), Streptococcus pneumonia (12.09±0.07, 22.00±0.08, and 15.86±0.05),Streptococcus pyogenes (14.51±0.64, 23.05±0.73, and 17.74±0.07). Streptococcusagalactiae (10.92±0.11, 18.96±0.11 and 18.01±0.09) and Klebsiella pneumoniae(10.70±0.05, 22.12±0.08, and 19.13±0.09). The metabolites of Bacillus subtilisexhibited significant activity against Streptococcus pyogenes (14.51±0.64).
Keyword :
Keywords: Bacillus subtilis, Secondary metabolites, Antibacterial, GC/MS