Abstract :
Isolated Neisseria species showed highest Chitinase hydrolysis activity on CAM therefore selected for solid state fermentation at 25℃. The extracted crude chitinase enzyme further used for the Chitinase activity by DNS method. The crude Chitinase were characterized by using different parameters such as temperature ranges from 25-50°c (optima-26°c), pH ranges from 5-9 (optima-pH 6.0), substrate concentration 0.2 % – 1.4 % (optima- 0.4 %), metal ions (inhibited by Kcl and increased by FeSO4).
Keyword :
Chtin, Acidic chitinase, Characterization.