Abstract :
This study was conducted to isolate and identify the biologically potential actinomycetes. Total of 70 cultures were isolated from different habitat of Gwalior region, Madhya Pradesh, India. All isolated actinomycetes evaluated for their ability to inhibit human pathogens, i.e. Microsporum canis, Microsporum gypseum, Microsporum fulvum, Tricophyton rubrum and Tricophyton mentagrophyte. All isolates were screened for their antifungal activity by agar well method against dermatophytes. After screening out of these isolates, only one isolate designated as AP-27 showed antifungal activity against Microsporum canis, Microsporum gypseum, and Tricophyton rubrum. Cultural, morphological and biochemical studies of isolate AP-27 classified it into Streptomyces spp. The 16S rRNA gene sequence of isolate AP-27 classified as Streptomyces griseus. This study proves that actinomycetes isolated from soil of Gwalior region have good antifungal activity against dermatophytes.
Keyword :
Soil actinomycetes; Antifungal activity; Dermatophytic fungi; secondary metabolite.