Abstract :
Abstract Background : Various VOCs coming from or absorbed by the Haemophilus influenzae cultures were identified using the GC-MS after the headspace sampling was done with the help of multibed sorption tubes. During bacteria cultivation, sampling was done at various time intervals to analyze the changes that occurred in VOC metabolism. The purpose of our study: Studying the bioactive volatiles produced by Haemophilus influenzae and Examining the antibacterial potential of the medicinal plants Citrullus colocynthis and Urtica dioica with Foeniculum vulgare. Materials and Methods: The Fresh of Citrullus colocynthis, Urtica dioica, and Foeniculum vulgare used in this present study was bought form a local market in Hilla city /Iraq. Each plant sample was ground in a mortar approximately weighing one hundred grams. Each plant powder 10g was extracted with ethanol extract of 100ml the solution was filtered and evaporated. The examination itself was performed using a GC–MS approach, and an Agilent 789 A instrument was used. The extracts were prepared and the sterile blotting paper disc (5 mm) was soaked in the diluted extract at two final concentrations; 50 ?L and100 ?L per disc. Results : GC-MS analysis of Haemophilus influenzae found thirteen bioactive compounds. Investigation effect of ethanolic extract of Citrullus colocynthis, Urtica dioica and Foeniculum vulgare comparison with Rifambin and Cefotoxime (standard antibiotics) as antibacterial activity against Haemophilus influenzae recorded 16.29±0.06, 10.89±0.03, and 12.36±0.03 respectively for Citrullus colocynthis while recorded 15.09±0.05, 13.08±0.04, and 09.73±0.03 respectively for Urtica dioica. Investigation effect of ethanolic extract of Foeniculum vulgare recorded 14.73±0.05, 10.94±0.03, and 09.64±0.04 respectively. Citrullus colocynthis 16.29±0.06 mm was very highly active against Haemophilus influenzae.
Keyword :
Keywords: Haemophilus influenzae, GC-MS, Citrullus colocynthis, Urtica dioica, Foeniculum vulgare, Antibacterial activity