Volume :
2
Issue :
3
Abstract :
A simple, specific, sensitive, precise and reproducible Reverse Phase High Performance Liquid Chromatography method has been developed for simultaneous estimation of Lovastatin and Niacin. Niacin and Lovastatin is Anti-hyperlipidemic Sustained Release drug. The determination was carried out by using symmetry C-18 column with ACN:0.05M Phosphate Buffer (PH4) (85:15) as the mobile phase and with the detection wavelength of 239,245 nm respectively. The flow rate is 0.6 ml/ min. The Retention time of Lovastatin, Niacin was 5.0 min and 3.2 min respectively. Linearity for the Niacin and Lovastatin were found in the range of 5-70 µgm and 5 - 50 µgm respectively. The limit of quantification for both drugs was found to be 30, 10 ng respectively. The recoveries of Niacin and Lovastatin were found to be in the range of 99.25-101.15% and 99.57-100.15%, respectively. The proposed method was validated suitably and can be used for routine analysis.