Abstract :
Background: Pseudomonas aeruginosa is Gram- Negative bacterium and one of causing of Hospital and community-acquired infections. Over and improper use of antibiotics leads to significant changes in microbial genetic ecology of the bacterium that leads to the spread of multidrug resistance which become now a global problem. The study aimed to determine the prevalence of Pseudomonas aeruginosa burn &wound infections and their antibiograms toward common commercial antibiotics.
Patients and Methods: Swabs were taken from patients with burn and wound infections. Specimens were examined microscopically as soon as possible (within one hour) by direct Gram-stained smears and indirectly by cultivation aerobically using suitable culture media. Bacterial isolates were diagnosed and confirmed using suitable diagnostic techniques. The antibiotics susceptibility was determined by the Kirby Bauer Disc diffusion method and the results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines2018.
Results: A total of 34 isolates of Pseudomonas aeruginosa were isolated from a burn and wound infections during a period of six months, from September 20l8 to March 20l9. Antibiogram of Pseudomonas indicatedthatmost of isolates were resistant to Ceftriaxone (94.1%), Ceftazidime (94.1%), Cefotaxime (91.2%), Amikacin(61.8%), piperacillin(52.9%) and to a lesser extent to Gentamicin(35.3% ), Aztreonam(29.4%), and Tobramycin(26.5%). On the other hand most of the isolates were sensitive to Norfloxacin(76.5%), Ciprofloxacin(85.3%), Meropenem and Imipenem(91.2%)..
Conclusion: Carbapenems and fluoroquinolone antibiotics appeared to be the most effective agent against P. aeruginosa isolates. On the other hand, cephalosporins were quite not effective against P.aeruginosa.
Keyword :
Human Type 1 diabetes (T1D); Coxsackie virus type B3 & B4; ELIZA; and Conventional PCR