Abstract :
Introduction: In chronic diseases in general and in diabetes mellitus and hypertension in particular, there is an important imbalance between antioxidant defenses and the production of free radicals due to reactive oxygen species, thus leading to an increase in oxidant stress markers. Therefore, this study was designed to evaluate the antioxydative and protective effects of aqueous and ethanolic extracts of trichilia emetica stem bark in albinos rats with alloxan-induced diabetes or adrenaline-induced hypertensive.
Methodology: Two different batches of albinos rats were used distinctly and including a control one of 3 rats for each batch so a test group of 21 rats. Diabetes was induced by injecting Alloxane® intraperitoneally for 7 days. Hypertension was induced by injecting Adrenaline® intraperitoneally for eight days and we used visitech BP 2000 tools to measure arterial pressure. The aqueous and ethanolic extracts of Trichilia emetica were prepared and the obtained mixture was homogenized using a magnetic stirrer for 24 hours. Rats received the above-mentioned extracts orally at doses of 100 and 200 mg/kg bw for 6 days.
Results: Aorta and heart activities of catalase were significantly increased. This same trend was observed with superoxide dismutase activity together with increasing AChE catalytic activity in vessels. Treatment of rats for seven days with extracts of Trichilia emetic and nifedipineproduced a significant decrease of antioxidative markers activities of catalase and SOD for aqueous and ethanolic extracts as well. This action was slightly above nifedipineone administrated at 10 and 20 mg/kg bw. For rats treated with Trichilia emetica or with diastabolwe registered a significant decrease of MDA and GSH concentration.
Conclusion: Our results showed that the extracts have the potential to reduce the rate of reactive oxygen species and free radicals in rats with alloxane-induced diabetes or adrenaline-induced hypertensive. However, further large studies are needed to investigate the specific action and particularity of Trichilia emetica that could justify these activities.
Keyword :
Antioxidative activities, Antihypertensive, Trichilia emetica, Alloxan.