Volume :
2
Issue :
2
Abstract :
A simple and selective HPLC method is described for the determination of Tizanidine and Nimesulide in tablet dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of 35 volumes of water and 65 volumes of Methanol with detection of 243nm. Linearity was observed in the range 20-100 µg/ml for Tizanidine (r2 =0.999) and 20-100 µg /ml for Nimesulide (r2 =0.999) for the amount of drugs estimated by the proposed methods were in good agreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.