Volume :

3

Issue :

1

Abstract :

A rapid high performance liquid chromatographic method has been development and validation for the estimation of Lercanidipine and Atenolol stimultaneously in combined dosage form. A kromosil C-8 column having dimensions of 4.6µmx250mm and particle size of 5µm in isocratic mode , with mobile phase containing a mixture of Di- Potassium Hydrogen Phosphate and Acetonitrile in ratio of (70:30v/v) (pH adjusted to pH 6.5±0.5 using phosphoric acid was used . Detection was done at 215 nm using PDA detector. The mobile phase was pumped at a flow rate of 1.0µl/min and injection volume is10µl. The selected chromatographic conditions were founds to effectively separate Lercandipine (Rt 3.8min)(2) and Atenolol (Rt: 6.1 min) having resolution of 7.8. The method was validated in terms of linearity, accuracy, precision, and specificity, limit of detection and limit of quantitation.