Abstract :
The objective of this work is to synthesis extracellular alkaline protease by Bacillus sp. using low cost agro
waste in solid state fermentation (SSF). The cost effective feed stocks like rice bran, wheat bran, cassava, saw dust
and oil cake were taken for alkaline protease production. Among this, rice bran was found to give maximum enzyme
production. Various nitrogen compounds like peptone, urea, yeast extract, ammonium sulfate and ammonium
chloride were evaluated as nitrogen source and among them peptone gave maximum enzyme production. Maximum
production of alkaline protease was achieved when initial moisture content and initial pH were 70 % and 10
respectively.
Keyword :
Rice bran, Bacillus sp., Solid State Fermentation, alkaline protease