Abstract :
The biological efficacy of the herbal drug is derived by aiming acute toxicity test on experimental animals.
Acute toxicity test on experimental animals is used in information regarding hazard feature and dispensation of the
drug. The acute toxicity study of Pet. ether, benzene, chloroform and methanol extracts for Aponogeton natans. Linn.
were evaluated in Wistar albino rats. All the extracts i.e Pet. ether, benzene, chloroform and methanol were
administered orally at the maximum dose of 2000mg/kg/p.o. The adverse toxic symptoms of the animals were
observed up to 24 hours. All the extracts were also tested at the dose of 2000 mg/kg/p.o for 14 days toxicity study.
The animals were sacrificed on the 15th day, the biochemical and blood parameters were measured. Vital organs
histopathalogical interpretations were pretended further. The results of the test groups administered with pet ether,
benzene, chloroform and methanol showed that, there were no adverse effects in the general behavior and mortality
when compared with the control group. Further, the test groups administered with pet ether, benzene, chloroform
and methanol did not showed any significant changes in food, water intake, body weight, kidney and liver function
test and hematological parameters. Acute toxicity screening revealed no adverse motor or neurological changes,
gastrointestinal tract disorder, respiratory or locomotors effect or changes in any of the animals, when the extracts
were administered at 2000mg/kg per day for 14 days.
Keyword :
Acute oral toxicity, Aponogeton natans Linn. (Engl & Krause), acute toxicity, hematological parameters, biochemical parameters.