Abstract :
Phytochemical study directed by antimicrobial activity testing of the root extract of Salvadora persica known as
“Siwak†was conducted to identify the antimicrobial components. Fresh roots were extracted with ethanol and
the total extract was fractionated with CHCl3 and EtOAc. The antimicrobial activity was trapped to the CHCl3
soluble fraction. Column chromatography followed by CPTLC resulted in the isolation of three active
compounds. In addition to the major compound benzyl isothiocyanate two new minor derivatives 3-methoxy
benzyl isothiocyanate (1) and 3-hydroxy benzyl isothiocyanate (2) were identified. Structures were elucidated
using different spectroscopic tools including UV, MS, 1D- and 2D-NMR. The isolated compounds were active
against gram positive, gram negative bacteria and fungi.
Key words: Salvadora persica; antimicrobial; MIC, benzyl isothocyanate derivatives
Keyword :
Salvadora persica; antimicrobial; MIC, benzyl isothocyanate derivatives