Abstract :
Abelmoschusenculentus (L.) does not have only great nutritional value but also possess some pharmacological
activities. This plant is useful for the treatment of genito-urinary disorders, chronic dysentery, curing ulcers and
gave relief from hemorrhoids. There are no significant levels of an anti-nutritional factor or endogenous toxins
reported in this plant to date. In this study,a new method developed to isolate the purest form DNA from the
okra leaves. The young leaves of Okra were collected into the centrifuge tube and several steps have taken to
isolate the DNA from the okra plant leaves. It is difficult to isolate genomic DNA from the green leaves of the
okra because the tissue of okra contains high mucilaginous acidic polysaccharides content. The DNA isolated
by this method was tested for yield, purity, and ability to be amplified by PCR. In this experiment sodium citrate
was used for the removal of mucilaginous acidic polysaccharides contain in Okra leaves. these give best result
in the highest yields of total genomic DNA.
Keyword :
AN EFFICIENT METHOD FOR THE ISOLATION OF PUREST FORM OF DNA FROM THE OKRA PLANT