Abstract :
A new stability indicating RP-HPLC method has been developed for the determination of Losartan and
Hydrochlorothiazide in tablets (UV detection at 235 nm). Hydrochlorothiazide and Losartan obey Beer-Lambert’s
law over the concentration range 1.0-300.3 μg/ml and 4.4.0-240.24 μg/ml respectively with regression equations y =
5980.5x+535.64 (r2 = 0.9999) and y = 17490x -3093.1 (r2 = 0.9997) for Losartan and Hydrochlorothiazide
respectively. The combined tablet formulation of Losartan and Hydrochlorothiazide was subjected to acidic, alkaline,
oxidation, thermal, photolytic and humidity degradations and the method was validated. The LOQ and LOD were
found to be 0.734 μg/ml and 0.232 μg/ml for Losartan whereas and 0.921 μg/ml and 0.304 μg/ml for
Hydrochlorothiazide respectively.
Keyword :
Losartan, Hydrochlorothiazide, RP-HPLC, stability-indicating and validation.