Abstract :
The synthetic 4-azasteroid dutasteride inhibits the type 1 and type 2 isoenzymes of 5-alpha-reductase in a selective and competitive manner. Men with symptomatic benign prostatic hyperplasia (BPH), which is characterized by an enlarged prostate, may use it. Orally administered dutasteride has a 60% bioavailability and is quickly absorbed. This work focuses on the most recent developments in analytical techniques for quantifying dutasteride in various biological samples (including human plasma), as well as in commercially available dose forms and bulk. High-performance thin-layer chromatography (HPTLC), gas chromatography in conjunction with tandem mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), and UV spectroscopy will all be thoroughly examined in this review. The matrix, stationary phase, mobile phase composition, detection wavelength, RF value, retention duration, detection limit (DL), carrier gas, and flow rate are just a few of the characteristics that will be covered.
Keyword :
Spectrophotometry, HPLC, HPTLC, LCMS, GCMS, Plasma