Abstract :
Introduction: This study was done to compare microorganisms around dental implants inserted in site of missing mandibular first molars and in subgingival plaque of patients with chronic periodontitis.
Materials and Method: This study comprised of 20 chronic periodontitis patients and 20 patients who received dental implants. Subgingival plaque and peri-implant biofilm were sampled in both groups which were evaluated using 454-prosequencing of bacterial V1 to V3 regions of 16S rDNA.
Results: The mean probing depth in group I was 6.8 mm and in group II was 2.4 mm, clinical attachment level in group I was 7.3 mm and in group II was 0 and bone loss in group I was 6.7 mm and in group II was 0. The most predominant microorganisms in group I was Catonella, Desulfovibrio, Mogibacterium, Peptostreptococcus, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythensis and Treponema denticola. The three most abundant OTUs linked with the implant were Pseudomonas, Leptotrichia hongkongensis and Granulicatella adiacens.
Conclusion: Comparison of subgingival biofilms in patients with chronic periodontitis and biofilms around dental implants revealed significant diversity. It was found that dental implants may alter the composition of microbiome.
Clinical significance: Knowledge about comparison of subgingival biofilms in patients with chronic periodontitis helps in taking preventive precautions for successful out cone of dental implants.
Keyword :
Subgingival biofilms, Microbiome, Implants.