Abstract :
A simple, Precise, accurate RP-HPLC method was developed and
validated for the estimation of cilnidipine in pharmaceutical dosage
forms. separation was achieved on Symmetry C18 column (4.6 x
150mm), with mobile phase consisting of ortho phosphoric acid buffer
pH 4 and Acetonitrile in 60:40, V/V. The flow rate was maintained at 1
ml/min and the analyte was monitored at 240nm wavelength. The
retention time for cilnidipine was found to be 2.35 min. The linearity of
the method was observed in the concentration range of 5-25ppm and
correlation coefficient was found to be 0.999. The percentage assay of
Cilnidipine was found to be 98.733%.
The method was robust and rugged as observed from insignificant
variation in the results of analysis by changes in flow rate and analysis
being performed on different days.
The method was validated for its accuracy, precision and system
suitability. The results obtained in the study were within the limits of
ICH guidelines and hence this method can be used for the routine
estimation of Cilnidipine in pharmaceutical dosage forms.
Keyword :
Cilnidipine, RP- HPLC, Validation etc.