Volume :

6

Issue :

2

Abstract :

A simple, rapid, accurate and precise LC-MS Compatible RP-HPLC method was developed and validated for the determination of Ticagrelor in bulk. Separation of the drug was achieved on Unisol C18 column (100 mm x 4.6 mm, 5 μ) as stationary phase with mobile phase consisting of ammonium acetate buffer pH 4.5 and acetonitrile in the ratio of 40: 60 V/V. The method showed a good linear response in the concentration range of 10-50 μg/mL with correlation coefficient of 0.99. The flow rate was maintained at 1.0 mL/min and effluents were monitored at 250 nm. The retention time was 3.88 min. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, solution stability, selectivity. The results obtained in the study were within the limits of ICH guidelines and hence this method can be used for the determination of Ticagrelor in pharmaceutical dosage forms.