Abstract :
Objective:In this study, levamisole, zinc and vitamin AD3E were used with enterotoxemia vaccine in sheep, in order to investigate their immunostimulant activities and also to make a comparison among them regarding their immunostimulatory properties.
Materials and Methods: For this purpose 40 sheep were divided into 4 groups, each group consisted of 10 sheep. Group I only vaccinated against enterotoxemia. In groups II, III and IV besides enterotoxemia vaccine, levamisole, zinc and vitamin AD3E were used respectively. Blood samples were taken on days 0 and 35 of the experiment. Serum levels of immunoglobulins (IgM, IgG, IgA and IgE) were determined using ELISA kits specific to sheep immunoglobulins. Hematological parameters and serum zinc levels were also determined.
Results:Levamisole-treated group showed a significant increase in serum level of IgM, and zinc-treated group demonstrated a significant increase in serum IgG level (P
Keyword :
Clostridium perfringens, enterotoxemia vaccination, immunoglobulins, immunostimulants