Abstract :
Introduction: Parasitic infections like Amoebiasis are endemic to most tropical and subtropical regions of developing countries like India. Among Entamoeba species, Entamoeba histolytica, the causative agent for amoebiasis, is highly pathogenic while the other species are considered as non-pathogenic and needs no medical treatment in most of the cases.Aim & Objective: To estimate the accurate prevalence of infection by different microscopically identical species of Entamoeba in human faecal samples by PCR based identification among patients attending a tertiary care hospital in Kolkata.Materials and Methods: A total of 1837 stool specimens in this study were collected from patients attending department of Calcutta School of Tropical Medicine for a period of 12 months. Stool samples were screened via microscopy for Entamoeba species identification followed by confirmation by conventional Polymerase chain reaction. Data were analyzed with Graph pad Prism 9.Results & Discussion: In the study, 91/1837 (4.95%) samples were microscopically positive for Entamoeba cyst or trophozoite and 81/1837 (4.41%) were Polymerase chain reaction positive for Entamoeba. E. moshkovskii infection (38.27%, 31/81) appeared to be the most predominant, followed by E. histolytica (19.75%, 16/81); E. dispar (9.88%, 08/81) and E. poleki (1.23%, 01/81) while 25/81(30.86%) belonged to other Entamoeba species.Conclusion: The results provide an important data for the clinician about the advantages of PCR over microscopy and also discriminate non-pathogenic Entamoeba species from the pathogenic E. histolytica to avoid unnecessary treatment with anti-amoebic drugs.
Keyword :
Entamoeba, Entamoeba histolytica, microscopy, Polymerase Chain reaction