Abstract :
Background: Limbal and amniotic stem cells are crucial for corneal regeneration, positioning them as essential components for ocular surface repair. Platelet-rich plasma (PRP) holds significant potential for promoting regeneration; however, its effects on the viability and proliferation of stem cells in vitro remain largely unexamined.Objective: This study seeks to assess the ability of PRP to improve the viability and proliferation of limbal and amniotic stem cells, which has important implications for corneal tissue engineering.Materials and Methods: The research studied PRP supplementation's effect on stem cell cultures, focusing on cell viability and proliferation. A quantitative analysis compared PRP-treated and control groups over five passages.Results: After 24 hours, limbal stem cells exposed to PRP exhibited a significantly higher viability rate of 90.60% compared to 83.40% in the control group (p = 0.001). Similarly, PRP-treated amniotic cells demonstrated 86.40% viability, compared to 81.00% in controls (p = 0.002). After 48 hours, viability in PRP-treated limbal cells rose to 93.80%, compared to 86.60% in controls (p < 0.001), while amniotic cell viability increased to 90.40% versus 85.80% in controls (p = 0.015). Proliferation rates also increased significantly in both cell types with PRP treatment. Limbal cells showed a proliferation rate of 202.55%, compared to 195.61% in controls (p < 0.001), and amniotic cells showed 173.43% versus 168.32% in controls (p < 0.001).Conclusion: PRP significantly enhances both the viability and proliferation of rabbit limbal and amniotic stem cells, highlighting its potential application in tissue engineering and corneal regeneration.
Keyword :
Platelet-rich plasma, Limbal stem cells, Amniotic stem cells, Cell viability, Cell proliferation, Tissue engineering, Corneal regeneration.