Abstract :
Aim: This article describes a novel, easy-to-use technique for using the dye exclusion experiment to assess the vitality of yeast cells. Methods: Yeast cells were propagated and suspended in a solution of trypan blue and phosphate buffered saline. After that, the suspension was put into the Neubauer chamber for analysis. Results: The living cells showed no color; that is, they repelled the dye, whereas the dead cells looked blue, since the cell membrane of dead cells allowed the entry of the dye. The viable (live) cells present in a suspension can be quantified following an exclusion test employed in this article. The outcome is based on the fact that intact cell membranes in living cells prevent the entry of certain dyes, such trypan blue. Conclusion: In this paper, we report a simple trypan blue dye-exclusion method to determine the viability of yeast cells.
Keyword :
yeast, dye exclusion assay, trypan blue, viability test.