Abstract :
In the present research work, in vitro anticancer potential of methanolic extract of some seed spices was evaluated via SRB assay. The anti-cancer activity was determined by the cytotoxic potential of test material at 100 µg/ml. Cells were allowed to grow for 24 h on 96-well flat bottom tissue culture plates and cells were further allowed to grow in the presence of test material for 48 h. Cell growth was terminated by addition of 50% (w/v) tricarboxylic acid and cells were stained with SRB dye. Excess dye was removed by washing with 1% (v/v) acetic acid and bound dye was dissolved in Tris buffer.OD was taken at 540 nm. Cuminum cyminum displayed in vitro cytotoxic effect against CNS human cancer cell line and Piper nigrum showed in vitro cytotoxic effect against human cancer cell line from colon origin. Further, isolation and characterization of active ingredients is required for the development of anticancer drugs.
Keyword :
Cuminum cyminum, Piper nigrum, SRB assay, In vitro cytotoxicity