Abstract :
In the present research work, in vitro anticancer potential of methanolic extract of kalonji and carom seeds was evaluated via SRB process. The anti-cancer activity was determined by the cytotoxic potential of test material at 100 µg/ml. Cells were allowed to grow for 24 h on 96-well flat bottom tissue culture plates and cells were further allowed to grow in the presence of test material for 48 h. Cell growth was terminated by addition of 50% (w/v) tricarboxylic acid and cells were stained with SRB dye. Excess dye was removed by washing with 1% (v/v) acetic acid and bound dye was dissolved in Tris buffer. OD was taken at 540 nm and growth inhibition of 70% or above was considered active for our bioassay purpose. Kalonji displayed in vitro cytotoxic effect against two human cancer cell lines from two different tissues and Trachyspermum ammi showed in vitro cytotoxic effect against four human cancer cell lines originated from four different tissues.
Keyword :
Nigella sativa, Trachyspermum ammi, SRB process, in vitro anticancer