Abstract :
Aim: To estimate the concentration of metal ions (Ni, Cr, Fe and Co), cytotoxicity and genotoxicity of buccal mucosal cell in patients being treated with fixed orthodontic appliances.
Materials and Methods: A total of 50 patients aged between 14-30 years of age were included in the study. Three samples of buccal cells were collected from each orthodontic patient at the following times: T (0) – before insertion of the appliance, T (1) – 3 months after insertion of the appliance, T (2) – 6 months after insertion of the appliance. Metal ion concentration of buccal cells was evaluated using atomic absorption spectrophotometer (AAS). The buccal cells were also evaluated for genotoxicity (DNA damage) and cytotoxicity (cellular viability) using alkaline comet assay and tryphan blue exclusion dye test respectively.
Results: It was noted that there was a significant (p
Keyword :
Metal Ions, Buccal cells, Cytotoxicity, Genotoxicity