Abstract :
Objective: To have in depth knowledge about the effects of nonselective and selective inhibitory and activated
agents on adenylyl cyclase in rat kidney.
Design: A variety of concentrations of pharmacological agents were prepared. They include nebularine, Ap3A,
forskolin, Ap4A and caffeine. Furthermore, effects of these agents were noted in relation to rat kidney adenylyl
activity.
Methods: Tissue of rat kidney was used in the process of preparation of crude extract. Activity of adenylyl cyclase in
connection with crude extract was observed. [2-H3] ATP was used as substrate which ultimately lead to the
formation of cAMP. Pharmacological agents with their prepared concentrations were tested. Prominent activator of
adenylyl cyclase, forskolin, was selected as a compound. Ap4A, caffeine, nebularine, and Ap3A were utilized for
comparison purpose.
Results: Adenylyl cyclase activity was at peak at 100 M forskolin as per concluded results. Nebularine inhibited
activity of enzyme when agent concentration enhanced up to 50 M where the inhibition started to stable. No
considerable effect on the enzyme activity in kidney tissue was observed when caffeine with 10 – 300 M on the of
adenylyl cyclase activity was used. No effect on adenylyl cyclase activity was noted when Ap3A over the
concentration range of 10 – 300 M was used. However, an inhibition effect on the enzyme activity was noted when
Ap4A with the concentration 100 M was used.
Conclusion: Role of cyclic nucleotides in metabolism control and cell-signaling is undeniably significant. It
stimulates inhibitors and activities of cyclase to make some likely physiological impacts. Foreskin being initiator of
adenylyl cyclase, Ap4A and nebularine are established to be latent inhibitors of cyclase, which signifies their
importance in curing schizophrenia, mania, seizure etc.
Key Words: Ap3A, Nucleotides, Caffeine, Forskolin, Ap4A, Nebularine. Adenylyl cyclase
Keyword :
Ap3A, Nucleotides, Caffeine, Forskolin, Ap4A, Nebularine. Adenylyl cyclase