Abstract :
Introduction: Ischemia is a worldwide scare and denotes decreased oxygen supply to various tissues of
our body. Ischemia modified albumin, a biomarker to diagnose cardiac ischemia has a role in oxidative
stress. Diabetes mellitus carbohydrate metabolic disorder is an oxidative stress condition. Total Thiol is an
antioxidant, which reduces the reactive oxygen species. There have been lot of research which proved the
association of diabetes with ischemia modified albumin. In this study we tried to compare the association
of ischemia modified albumin with prediabetes as well as diabetes.
Materials and Methods: After obtaining the ethical approval from institutional ethics committee, 120
serum samples were collected (40 - normal healthy subjects, 40 - prediabetes, 40 - diabetes mellitus) and
analyzed for ischemia modified albumin using spectrophotometric Co(II)-albumin binding assay and total
Thiol was measured using spectrophotometric method. Glycated hemoglobin, lipid profile values were
obtained from laboratory information system. Data were compiled and appropriate statistical tests were
used wherever necessary. p value
Result: This study reported decreased levels of ischemia modified albumin and increased levels of total
thiol in both prediabetes as well as diabetes mellitus as compared to normal control. Significant positive
correlation between ischemia modified albumin and total thiol was obtained.
Conclusion: Oxidative stress maker IMA can be neutralized by an anti-oxidant total thiol. People consume
antioxidant to prevent progression of chronic diseases. Extra supplementation of antioxidant may not be
much helpful in cases where naturally antioxidants are produced due to the diseases. Thus measuring both
helps in prevention of extra supplementation of anti-oxidants.
Keyword :
Ischemia modified albumin, Total thiol, Prediabetes, Diabetes mellitus, Lipid profile, Glycated hemoglobin.