Abstract :
Introduction: RBC histogram with erythrocyte indices provide clue to the diagnosis of anemia .Along with Peripheral smear it is used to interpret the cause of anemia.
Aim: To correlate peripheral smear findings with RBC histogram patterns and RBCs indices to provide a better approach in the diagnosis of anemia and analyse their limitations.
Materials and Method: 500 anemic patients with haemoglobin levels of less than 11.5gm% on EDTA sample by Beckman coulter LH-780 were included in the present study, leukemia being excluded. An impression was made by examining Leishman stained peripheral smear without referring to the histogram. The RBC histogram and the RBC indices were analysed and the anemia categorised. The correlation between the diagnosis made by Peripheral smear Vs RBC histogram, indices was analysed using Cramer’s V (0.523) and Kappa statistics for agreement between the two methods.
Results: The 500 cases included 21.8%, 46%, 8.8%, 17.6% and 5.8% cases of normocytic normochromic, microcytic hypochromic, macrocytic, dimorphic and haemolytic anemia respectively by peripheral smear examination. Analysis by erythrocyte indices and histogram showed similar findings except in macrocytic, dimorphic and hemolytic anemia which were 15.6%, 15.8% and 0.8% respectively. The correlation between the diagnosis made by the two methods was statistically significant, p
Keyword :
Histogram, Erythrocyte Indices, Peripheral Smear, Anemia, Hemolytic Anemia