Article PDF :

Veiw Full Text PDF

Article type :

Original Article

Author :

Archana Bora, PK Khatri, Aruna Solanki, RS Parihar, AK Chandora

Volume :

3

Issue :

4

Abstract :

Introduction: With increase in Beta – Lactamases producing microbial infections there is need for rapid method for identification of different types of Beta Lactamases enzyme production & their coexistence in clinical isolates. Vitek – 2 compact play very crucial role in detection of different types of Beta- Lactamases & their coexistence. Materials & Methods: 240 clinical specimen like Urine, Tracheal Secretion, Sputum, pus & Stool were isolated from clinical samples of OPD & IPD Patients of Tertiary Care Hospitals attached to Dr. S.N. Medical College, Jodhpur. Identification of Clinical isolates & detection of Betalactamase enzymes with their antibiotic susceptibility pattern was done by automated Vitek – 2 compact System. Results: 146 (60.83%) E. coli, 67 (27.91%) Klebsiella, 22 (9.17%) Enterobacter, 2 (0.83%) Citrobacter & 3 (1.25%) Serratia spp. were included in present study. Out of 240 isolates 195 (81.25%) were enzymes producers & 45(8.75%) were non-enzyme producer. Of the 195 isolates 147(61.25) were single enzyme producer & 48(20%) isolates were multiple enzyme producers. 105(44%) isolates shows only ESBL enzyme production, 26 (11%) isolates shows AMPc enzyme & 16(7%) isolates shows carbapenemase enzymes production. Of the 48 isolates producing mixed enzymes 45.83% shows ESBL AMPc and ESBL CARBAPENEMASE each and 16.66% shows all enzymes (ESBL AMPc CARBAPENEMASE) production. Isolates producing ESBL are maximally resistant to Cefepime followed by Cefuroxime, & Ceftriaxone. Organism producing AMP care maximally Resistant to Cefuroxime followed by Trimethoprim - Sulfmethoxazole, Piperacillin-Tazobactum, Ampicillin. Organism producing CARBAPENEMASE are maximally Resistant to Ampicillin, Cefipime followed by Cefuroxime Sulbactum, and Amoxicillin Clavulinic acid. Organism producing mixed enzymes are maximally resistant to Cefuroxime, followed by Amoxicillin Clavulinic acid and Ciprofloxacin. Conclusion: The study show that Betalactamase producing microbial organism are very common in Hospital environment and responsible for majority of infections. Vitek-2 compact is found to be rapid and reliable method to identify Betalactamase and their co-existence. It is better than conventional method.