Abstract :
Four Streptomyces strains isolated from the soil of Western Ghats of India producing Amphotericin B were subjected to random mutagenesis by UV irradiations for yield improvement. The mutants were then screened for their Amphotericin B concentrations and their antifungal activity by agar well diffusion method. Two mutants demonstrated 13% and 16% higher concentration than the isolated strains respectively. Thus mutation can be a very useful tool to minimize production cost with the same benefits.
Keyword :
Amphotericin B, UV mutation, Streptomyces, strain improvement