Abstract :
Aim: In this study, we aimed to isolate and identify endophytic fungi from the Ocimum sanctum plant, commonly known as Holy Basil and Hindi name- Tulsi.
Objective: 1. To investigate the endophytic mycobiota from Ocimum sanctum. 2. To isolate and identify some endophytic fungi in selected medicinal plant. 3. To study phenotypic characteristics of endophytic fungi in selected medicinal plant.
Methods: Methods employed for the isolation and identification of endophytic fungal strains encompassed a multi-step approach, involving surface sterilization, tissue maceration, and subsequent fungal isolation. Morphological and microscopic characterization were utilized to identify the isolated fungal strains. As a result, a total of five distinct endophytic fungal strains were successfully isolated and identified.
Result: The results revealed a high diversity of endophytic fungi associated with Ocimum sanctum. Furthermore, several potential bioactive compounds were detected in the isolated fungi, further emphasizing their importance.
Conclusion: This study contributes to the knowledge of endophytic fungi associated with Ocimum sanctum and provides a foundation for further investigation into their biotechnological potential.
Keyword :
Endophytic fungi, Ocimum sanctum, Isolation, Identification, Bioactive compounds