Efficacy of low-level laser therapy enhances the rate of canine retraction by increasing β glucoronidase and pentraxin – 3 in gingival crevicular fluid: A clinical study


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Article type :

Original Article

Author :

Unnati Dinesh Parekh, Shraddha Shetti, Jiwanasha Manish Agrawal, Manish Agrawal, Sangamesh Fulari, Someshwar Golgire

Volume :

8

Issue :

3

Abstract :

Aim: The aim of the study was to evaluate the efficacy of low-level laser therapy in enhancing the rate of orthodontic tooth movement using β glucoronidase and pentraxin-3 (TNF) biomarkers in gingival crevicular fluid. Materials and Methods: A split-mouth design was used in 16 subjects (8 experimental and 8 controlled) aged 14 to 25 years, who’s maxillary first premolars were extracted. A gallium-aluminium-arsenide semiconductor diode laser (wavelength, 810 nm; energy density, 10 J/cm2; power output, 100 mW) delivered low-level laser therapy to the experimental canine undergoing distalization at 10 points. The control canine was distalized without low-level laser therapy. The experimental and control canines were distalized using a force of 150 g provided by nickel-titanium closed coil springs. Gingival crevicular fluid was collected at 5 time points from the control and experimental sides, and the levels of β glucoronidase and pentraxin-3 were analysed by enzyme-linked immuno-sorbent assay (ELISA). The rate of canine retraction was calculated by using ortho analyser software. Results: Increased levels of β glucoronidase and pentraxin-3 were observed in the experimental canines compared with the control canines (P Conclusion: Low level laser therapy in adjunct with continuous and light forces leads to increase in β glucoronidase and pentraxin-3 leading to accelerated orthodontic tooth movement. Clinical Significance: Low-level laser therapy fastens the rate of canine retraction thus accelerating the orthodontic tooth movement. The levels of β glucoronidase and Pentraxin 5 are increased significantly after laser irradiation which aids in accelerating orthodontic tooth movement.

Keyword :

Accelerated orthodontics, Gingival crevicular fluid, Low level laser therapy, Rate of retraction