A simple, precise, accurate RP-HPLC method was developed and validated
for the estimation of Dasatinib in pharmaceutical dosage forms. An
Cosmicsil BDS C18 column (150 mm x 4.6 mm), 5 Âµ particle size was used
as stationary phase with mobile phase consisting of Phosphate buffer: a
mixture of acetonitrile and methanol in the ratio of 50:50, v/v. The flow
rate was maintained at 1 mL/min and effluents were monitored at 315
nm. The retention time was 6.4675 min. The linearity of the method was
observed in the concentration range of 20-60 Âµg/mL with correlation
coefficient of 0.999. The percentage assay of Dasatinib was 100.10%. The
method was validated for its accuracy, precision and system suitability.
The results obtained in the study were within the limits of ICH guidelines
and hence this method can be used for the estimation of Dasatinib in
pharmaceutical dosage forms.
Dasatinib, Cosmicsil BDS C18 column, RP-HPLC, Validation