Abstract :
Abstract Metabolites are small molecules participating in metabolicreactions, which are necessary for cellular function, maintenance andgrowth. Typically, metabolites range from 50 to 1500 Da, while theirconcentrations span several orders of magnitude. The metabolome is highlydynamic, time-dependent, and metabolites are sensitive to manyenvironmental conditions. Streptococcus pyogenes has several virulencefactors that enable it to attach to host tissues, evade the immune response,and spread by penetrating host tissue layers. A carbohydrate-based bacterialcapsule composed of hyaluronic acid surrounds the bacterium, protecting itfrom phagocytosis by neutrophils. In addition, the capsule and severalfactors embedded in the cell wall, including M protein, lipoteichoic acid, andprotein F (SfbI) facilitate attachment to various host cells. Several of thediscriminant compounds, notably pyrazine compounds, are normally absentin healthy human exhaled breath but can be found in the Streptococcuspyogenes cultures. That these compounds are typically absent suggests that anumber of these candidate biomarkers are not typically produced by othermembers of the oropharyngeal microbial community.
Keyword :
Keywords: Streptococcus pyogenes, Secondary metabolites, Antibacterial, GC/MS