Abstract :
Indigenous plants in Northern border region of Saudi Arabia are still virgins; the majority of them are still not
markedly investigated for their phytoconstituents and/or their endophytic metabolites. The present work deals with
the isolation, identification of endophytic fungal strains and investigation of their antimicrobial and cytotoxic
activities from two indigenous plants, Polygonum aviculare and Achillea fragrantissima, three fungal strains have
been isolated and identified as Pseudeurotium oval, Aspergillus sydowii, and Trichoderma longibrachiatum. These
fungal strains were cultivated on solid nutrient media (rice media), their secondary metabolites were extracted and
fractionated. The antimicrobial and Cytotoxic activities of these fractions were evaluated. Sub-fractions of both A.
sydowii, and T. longibrachiatum showed a comparable antimicrobial activities while those of P. oval were inactive.
In addition, Sub-fractions of both A. sydowii, and T. longibrachiatum showed a promising cytotoxic activities
against three Mammalian cancer cell lines: MCF-7 cells (human breast cancer cell line), HepG-2 cells (human
Hepatocellular carcinoma) and HCT-116 (colon carcinoma) while the most promising results obtained from T.
longibrachiatum sub-fractions.
Key words: Achillea fragrantissima, Polygonum aviculare, Pseudeurotium oval, Aspergillus sydowii, and
Trichoderma longibrachiatum, Cytotoxic and antimicrobial activity
Keyword :
Achillea fragrantissima, Polygonum aviculare, Pseudeurotium oval, Aspergillus sydowii, and Trichoderma longibrachiatum, Cytotoxic and antimicrobial activity