Abstract :
Antioxidant assay was performed to count the total phenolic content in coelomic fluid of
Eisenia fetida. The worms were cultured in lab until they became sexually mature. The study focused
on total phenolic compound determination using two solvents in a single species of earthworm. It
was analysed that the extraction of phenolic compound with 85% ethanol yielded high phenol
content, 208.6 mg GAE\L Eisenia fetida. Extraction with 85% methanol on the other hand, yielded
189.1 mg GAE\L. Eisenia fetida ethanolic extract in the sample manifested high total phenolic content
than methanolic extract. Phenols are more readily soluble in ethanol and safe for human
consumption. Methanol is used as solvent for low molecular weight polyphenols. The study
concludes that ethanol extract of Eisenia fetida coelomic fluid contains significant amount of
phenols. Phenols have an antioxidant effect and can be used as natural antioxidants for curing
ailments related with inflammation and oxidative stress. This study will help in bringing attention
towards the therapeutic utility of earthworms.
Keyword :
Antioxidant activity, Coelomic fluid, Cold shock treatment, Ethanol, Gallic acid.